diff --git a/TeachingModule/AnalysisMSData_FragPipe.qmd b/TeachingModule/AnalysisMSData_FragPipe.qmd index 8cf4b82..88e53ce 100644 --- a/TeachingModule/AnalysisMSData_FragPipe.qmd +++ b/TeachingModule/AnalysisMSData_FragPipe.qmd @@ -22,11 +22,9 @@ What is FTP, and what is its functionality? ::: -For downloading the data, we will use the **Proteomics Sandbox Application** on UCloud. This platform allows us to access the necessary storage capacity as well as the computational power required to execute this process. +To download the data, we will use the **Proteomics Sandbox Application** on UCloud. This platform provides the necessary storage capacity and computational power to perform this process. -The **Proteomics Sandbox Application** is a virtual environment that includes multiple software tools, including **FragPipe** for analyzing proteomics data. - -You can find the **Proteomics Sandbox Application** on UCloud [here](https://cloud.sdu.dk/app/jobs/create?app=proteomics). +The **Proteomics Sandbox Application** is a virtual environment that includes various software tools, such as **FragPipe**, for analyzing proteomics data. First, we will download the data for the sample files to be used in **FragPipe**. Then, we will launch **FragPipe** to run the first analysis of the data. Before doing so, we have some questions regarding **FragPipe** and its usability: @@ -34,20 +32,19 @@ First, we will download the data for the sample files to be used in **FragPipe** What is FragPipe, and what are its applications? ::: - ::: {.question} -If FragPipe were not used for this part of the teaching module, which alternative software tools could be employed? Please provide a few examples. +If FragPipe was not used for this part of the teaching module, which alternative software tools could be employed? Please provide a few examples. ::: - ::: {.question} What are the benefits of using FragPipe? ::: - Now that we know what we want to do and why, it is time to start the **Proteomics Sandbox** application, or job. Simple analyses in **FragPipe** may only require 8 GB of RAM, while large-scale or complex analyses may require 24 GB of memory or more ([FragPipe Documentation](https://fragpipe.nesvilab.org/docs/tutorial_fragpipe.html#:~:text=FragPipe%20runs%20on%20Windows%20and,24%20GB%20memory%20or%20more.)), which is why we will allocate 24 GB for this exercise. +You can access the **Proteomics Sandbox Application** on UCloud [here](https://cloud.sdu.dk/app/jobs/create?app=proteomics&version=Oct2024). + In UCloud, the settings should look like this: ![](images/TeachingModuleInstructions/UCloud_settings.PNG){width=750 fig-align="center"} @@ -83,7 +80,9 @@ At the bottom of the page, you will find the FTP-server address where the data i Please locate the address. ::: -Click on the "Dataset FTP location" link. +::: {.callout-tip} +Click on the **Dataset FTP location** link. +::: ::: {.question} We now have access to the data stored on the FTP server. Please provide a brief description of the contents of the folder on the FTP server. @@ -96,7 +95,10 @@ To download one sample file from each of the Plex Sets, we will need these URLs {{< include ./urls.txt >}} ``` -(You can also download this list [here](https://github.com/hds-sandbox/proteomics-sandbox/blob/webpage/TeachingModule/urls.txt)). +::: {.callout-tip collapse="true"} +## Tip to Download of Data +You can also download this list [here](https://github.com/hds-sandbox/proteomics-sandbox/blob/webpage/TeachingModule/urls.txt). +::: ::: {.callout-note} We recommend to open this material inside **Proteomics Sandbox** to be able to copy & paste or download the file directly into the environment. @@ -119,6 +121,7 @@ Now that FragPipe is launched, we need to configure the settings before running ::: {.callout-note} Some of the information you will need in this section can be found in **Supplementary Information** to the study. Open the **Supplementary Information** and go to page 25, **Supplementary Methods**. ::: + Go to the `Workflow` tab to set up the workflow for the analysis and import the data you just downloaded. ::: {.question} diff --git a/_quarto.yml b/_quarto.yml index 902f81c..a3aa25a 100644 --- a/_quarto.yml +++ b/_quarto.yml @@ -7,23 +7,33 @@ website: left: - text: Introduction href: index.qmd - - text: Getting Started - href: gettingstarted.qmd - - text: Setup - href: setup.qmd - - text: ColabFold - href: colabfold.qmd - - text: Course Materials - href: coursematerials.qmd - - text: Teaching Module - href: teachingmodule.qmd - - text: Contributors - href: contributors.qmd + - sidebar: + id: ProteomicsSidebar + title: "Navigation" + style: "docked" + background: "light" + collapse-level: 2 + contents: + - id: ProteomicsSandboxApp + title: "Proteomics Sandbox App" + contents: + - text: Getting Started + href: gettingstarted.qmd + - text: Setup + href: setup.qmd + - text: ColabFold + href: colabfold.qmd + - text: Course Materials + href: coursematerials.qmd + - text: Teaching Module + href: teachingmodule.qmd + - text: Contributors + href: contributors.qmd format: html: theme: - light: [materia, css/materialight.scss] - dark: darkly + light: [materia, css/materialight.scss] + dark: darkly toc: true css: css/styles.css