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Support for --samples option in eventalign #74

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a-slide opened this issue Dec 15, 2020 · 5 comments
Closed

Support for --samples option in eventalign #74

a-slide opened this issue Dec 15, 2020 · 5 comments

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@a-slide
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a-slide commented Dec 15, 2020

Hi @hasindu2008

We use the --samples option in nanopolish eventalign to extract accurate signal parameters at kmer level in Nanocompore for RNA modification detection. It appears that the option is not yet available in fc5 eventalign. I know that it considerably increase the size of the file but we normally directly pipe into our pipeline.

Would you consider adding it ?

Thanks

@hasindu2008
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Yes, I would soon add this --samples option. Adding it to the existing DNA alignment is quite easy, I will investigate RNA alignment as I mentioned in the other response and let you know.

@a-slide
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a-slide commented Dec 16, 2020

Thanks

@hasindu2008
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@a-slide Now f5c dev branch supports --samples for DNA eventalign and the output formatting is parallelised for much faster multi-threaded performance.

@a-slide
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a-slide commented Jan 22, 2021

I can confirm it works for both DNA and RNA
Thanks @hasindu2008

By the way I pushed a singularity container with the dev version of f5 in case you are interested
containers: https://cloud.sylabs.io/library/aleg/default/f5c
recipes: https://github.com/a-slide/singularity/tree/main/f5c

@hasindu2008
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@a-slide
Thank you very much for testing and thanks for creating those singularity containers!

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