diff --git a/.changelog/18547.txt b/.changelog/18547.txt
new file mode 100644
index 00000000000..eaac1d3a5a1
--- /dev/null
+++ b/.changelog/18547.txt
@@ -0,0 +1,3 @@
+```release-note:enhancement
+resource/aws_datasync_location_s3: Add `agent_arns` argument
+```
\ No newline at end of file
diff --git a/aws/resource_aws_datasync_location_s3.go b/aws/resource_aws_datasync_location_s3.go
index 37723572fbc..8fa5bf11f88 100644
--- a/aws/resource_aws_datasync_location_s3.go
+++ b/aws/resource_aws_datasync_location_s3.go
@@ -29,6 +29,12 @@ func resourceAwsDataSyncLocationS3() *schema.Resource {
 				Type:     schema.TypeString,
 				Computed: true,
 			},
+			"agent_arns": {
+				Type:     schema.TypeSet,
+				Optional: true,
+				ForceNew: true,
+				Elem:     &schema.Schema{Type: schema.TypeString},
+			},
 			"s3_bucket_arn": {
 				Type:         schema.TypeString,
 				Required:     true,
@@ -97,6 +103,10 @@ func resourceAwsDataSyncLocationS3Create(d *schema.ResourceData, meta interface{
 		Tags:         tags.IgnoreAws().DatasyncTags(),
 	}
 
+	if v, ok := d.GetOk("agent_arns"); ok {
+		input.AgentArns = expandStringSet(v.(*schema.Set))
+	}
+
 	if v, ok := d.GetOk("s3_storage_class"); ok {
 		input.S3StorageClass = aws.String(v.(string))
 	}
@@ -168,15 +178,14 @@ func resourceAwsDataSyncLocationS3Read(d *schema.ResourceData, meta interface{})
 		return fmt.Errorf("error parsing Location S3 (%s) URI (%s): %s", d.Id(), aws.StringValue(output.LocationUri), err)
 	}
 
+	d.Set("agent_arns", flattenStringSet(output.AgentArns))
 	d.Set("arn", output.LocationArn)
-
 	if err := d.Set("s3_config", flattenDataSyncS3Config(output.S3Config)); err != nil {
 		return fmt.Errorf("error setting s3_config: %s", err)
 	}
-
+	d.Set("s3_storage_class", output.S3StorageClass)
 	d.Set("subdirectory", subdirectory)
 	d.Set("uri", output.LocationUri)
-	d.Set("s3_storage_class", output.S3StorageClass)
 
 	tags, err := keyvaluetags.DatasyncListTags(conn, d.Id())
 
diff --git a/aws/resource_aws_datasync_location_s3_test.go b/aws/resource_aws_datasync_location_s3_test.go
index e38060a8c16..f6262a0eed2 100644
--- a/aws/resource_aws_datasync_location_s3_test.go
+++ b/aws/resource_aws_datasync_location_s3_test.go
@@ -96,10 +96,12 @@ func TestAccAWSDataSyncLocationS3_basic(t *testing.T) {
 				Config: testAccAWSDataSyncLocationS3Config(rName),
 				Check: resource.ComposeTestCheckFunc(
 					testAccCheckAWSDataSyncLocationS3Exists(resourceName, &locationS31),
+					resource.TestCheckResourceAttr(resourceName, "agent_arns.#", "0"),
 					testAccMatchResourceAttrRegionalARN(resourceName, "arn", "datasync", regexp.MustCompile(`location/loc-.+`)),
 					resource.TestCheckResourceAttrPair(resourceName, "s3_bucket_arn", s3BucketResourceName, "arn"),
 					resource.TestCheckResourceAttr(resourceName, "s3_config.#", "1"),
 					resource.TestCheckResourceAttrPair(resourceName, "s3_config.0.bucket_access_role_arn", iamRoleResourceName, "arn"),
+					resource.TestCheckResourceAttrSet(resourceName, "s3_storage_class"),
 					resource.TestCheckResourceAttr(resourceName, "subdirectory", "/test/"),
 					resource.TestCheckResourceAttr(resourceName, "tags.%", "0"),
 					resource.TestMatchResourceAttr(resourceName, "uri", regexp.MustCompile(`^s3://.+/`)),
diff --git a/website/docs/r/datasync_location_s3.html.markdown b/website/docs/r/datasync_location_s3.html.markdown
index 7c20aef3921..61b3ebe0847 100644
--- a/website/docs/r/datasync_location_s3.html.markdown
+++ b/website/docs/r/datasync_location_s3.html.markdown
@@ -27,6 +27,7 @@ resource "aws_datasync_location_s3" "example" {
 
 The following arguments are supported:
 
+* `agent_arns` - (Optional) A list of DataSync Agent ARNs with which this location will be associated.
 * `s3_bucket_arn` - (Required) Amazon Resource Name (ARN) of the S3 Bucket.
 * `s3_config` - (Required) Configuration block containing information for connecting to S3.
 * `s3_storage_class` - (Optional) The Amazon S3 storage class that you want to store your files in when this location is used as a task destination. [Valid values](https://docs.aws.amazon.com/datasync/latest/userguide/create-s3-location.html#using-storage-classes)