diff --git a/internal/service/ec2/ec2_instance_test.go b/internal/service/ec2/ec2_instance_test.go
index cf3d952ad998..2c75b02a9402 100644
--- a/internal/service/ec2/ec2_instance_test.go
+++ b/internal/service/ec2/ec2_instance_test.go
@@ -5551,6 +5551,46 @@ func defaultSubnetCount(ctx context.Context, t *testing.T) int {
 	return len(subnets)
 }
 
+func TestAccEC2Instance_basicWithSpot(t *testing.T) {
+	ctx := acctest.Context(t)
+	var v ec2.Instance
+	resourceName := "aws_instance.test"
+	rName := sdkacctest.RandomWithPrefix(acctest.ResourcePrefix)
+
+	resource.ParallelTest(t, resource.TestCase{
+		// No subnet_id specified requires default VPC with default subnets.
+		PreCheck:                 func() { acctest.PreCheck(ctx, t) },
+		ErrorCheck:               acctest.ErrorCheck(t, ec2.EndpointsID),
+		ProtoV5ProviderFactories: acctest.ProtoV5ProviderFactories,
+		CheckDestroy:             testAccCheckInstanceDestroy(ctx),
+		Steps: []resource.TestStep{
+			{
+				Config: testAccInstanceConfig_basicWithSpot(rName),
+				Check: resource.ComposeTestCheckFunc(
+					testAccCheckInstanceExists(ctx, resourceName, &v),
+					resource.TestCheckResourceAttrSet(resourceName, "spot_instance_request_id"),
+					resource.TestCheckResourceAttr(resourceName, "instance_lifecycle", "spot"),
+					resource.TestCheckResourceAttr(resourceName, "instance_market_options.#", "1"),
+					resource.TestCheckResourceAttr(resourceName, "instance_market_options.0.%", "2"),
+					resource.TestCheckResourceAttr(resourceName, "instance_market_options.0.market_type", "spot"),
+					resource.TestCheckResourceAttr(resourceName, "instance_market_options.0.spot_options.#", "1"),
+					resource.TestCheckResourceAttr(resourceName, "instance_market_options.0.spot_options.0.%", "4"),
+					resource.TestCheckResourceAttr(resourceName, "instance_market_options.0.spot_options.0.instance_interruption_behavior", "terminate"),
+					resource.TestCheckResourceAttrSet(resourceName, "instance_market_options.0.spot_options.0.max_price"),
+					resource.TestCheckResourceAttr(resourceName, "instance_market_options.0.spot_options.0.spot_instance_type", "one-time"),
+					resource.TestCheckResourceAttr(resourceName, "instance_market_options.0.spot_options.0.valid_until", ""),
+				),
+			},
+			{
+				ResourceName:            resourceName,
+				ImportState:             true,
+				ImportStateVerify:       true,
+				ImportStateVerifyIgnore: []string{"user_data_replace_on_change"},
+			},
+		},
+	})
+}
+
 // testAccLatestAmazonLinuxPVEBSAMIConfig returns the configuration for a data source that
 // describes the latest Amazon Linux AMI using PV virtualization and an EBS root device.
 // The data source is named 'amzn-ami-minimal-pv-ebs'.
@@ -8849,3 +8889,19 @@ resource "aws_instance" "test" {
 }
 `, rName))
 }
+
+func testAccInstanceConfig_basicWithSpot(rName string) string {
+	return acctest.ConfigCompose(
+		acctest.ConfigLatestAmazonLinux2HVMEBSARM64AMI(),
+		acctest.AvailableEC2InstanceTypeForRegion("t4g.nano"),
+		fmt.Sprintf(`
+resource "aws_instance" "test" {
+  ami = data.aws_ami.amzn2-ami-minimal-hvm-ebs-arm64.id
+  instance_market_options {}
+  instance_type = data.aws_ec2_instance_type_offering.available.instance_type
+  tags = {
+    Name = %[1]q
+  }
+}
+`, rName))
+}