diff --git a/requirements_dev.txt b/requirements_dev.txt
index 56ec1efc..420e5c50 100644
--- a/requirements_dev.txt
+++ b/requirements_dev.txt
@@ -18,6 +18,6 @@ coverage[toml]
 pytest-cov>=2.5.1
 nbval>=0.9.6
 # Ref https://github.com/mhammond/pywin32/issues/1709
-pywin32==300 ; sys_platform == 'win32'
+pywin32==301 ; sys_platform == 'win32'
 # Code quality assurance
 pre-commit>=2.9.0