diff --git a/sci-biology/cutadapt/Manifest b/sci-biology/cutadapt/Manifest
new file mode 100644
index 00000000000..9b1b4fa206d
--- /dev/null
+++ b/sci-biology/cutadapt/Manifest
@@ -0,0 +1 @@
+DIST cutadapt-4.9.tar.gz 248837 BLAKE2B a95e510801572046689899315283d958933b625a10f57177811bbeae020b7a8f09c919cd15adabac64adbf550cdd901e078cd76cebdc0a1bbf80223584291242 SHA512 332de05a0fd11c07749ee58c171d79699776cae4a0338b7586f9e2939fb9f313b1647388bb4f060b7603e80b94b5b0da54a5e825fab862d6bf3c45b0c02fda0b
diff --git a/sci-biology/cutadapt/cutadapt-4.9.ebuild b/sci-biology/cutadapt/cutadapt-4.9.ebuild
new file mode 100644
index 00000000000..cd1a8fe7cf5
--- /dev/null
+++ b/sci-biology/cutadapt/cutadapt-4.9.ebuild
@@ -0,0 +1,28 @@
+# Copyright 1999-2020 Gentoo Authors
+# Distributed under the terms of the GNU General Public License v2
+
+EAPI=8
+
+PYTHON_COMPAT=( python3_{10..13} )
+DISTUTILS_USE_PEP517=setuptools
+
+inherit distutils-r1 pypi
+
+DESCRIPTION="Adapter trimming and other preprocessing of high-throughput sequencing reads"
+HOMEPAGE="https://github.com/marcelm/cutadapt/"
+SRC_URI="$(pypi_sdist_url "${PN^}" "${PV}")"
+
+LICENSE="MIT"
+SLOT="0"
+KEYWORDS="~amd64"
+
+IUSE="test"
+DEPEND="
+	>=dev-python/xopen-1.6.0[${PYTHON_USEDEP}]
+	>=dev-python/dnaio-1.2.0[${PYTHON_USEDEP}]
+"
+RDEPEND="
+	${DEPEND}
+	test? ( dev-python/pytest )
+"
+distutils_enable_tests pytest
diff --git a/sci-biology/cutadapt/metadata.xml b/sci-biology/cutadapt/metadata.xml
new file mode 100644
index 00000000000..028038de8c8
--- /dev/null
+++ b/sci-biology/cutadapt/metadata.xml
@@ -0,0 +1,12 @@
+<?xml version='1.0' encoding='UTF-8'?>
+<!DOCTYPE pkgmetadata SYSTEM "http://www.gentoo.org/dtd/metadata.dtd">
+<pkgmetadata>
+	<maintainer type="project">
+		<email>sci@gentoo.org</email>
+		<name>Gentoo Science</name>
+	</maintainer>
+	<upstream>
+		<remote-id type="pypi">cutadapt</remote-id>
+		<remote-id type="github">marcelm/cutadapt</remote-id>
+	</upstream>
+</pkgmetadata>