diff --git a/erpnext/selling/doctype/sales_order/test_sales_order.py b/erpnext/selling/doctype/sales_order/test_sales_order.py
index 261566ec001e..375accd65b68 100644
--- a/erpnext/selling/doctype/sales_order/test_sales_order.py
+++ b/erpnext/selling/doctype/sales_order/test_sales_order.py
@@ -2156,13 +2156,14 @@ def make_sales_order(**args):
 	return so
 
 
-def create_dn_against_so(so, delivered_qty=0):
+def create_dn_against_so(so, delivered_qty=0, do_not_submit=False):
 	frappe.db.set_single_value("Stock Settings", "allow_negative_stock", 1)
 
 	dn = make_delivery_note(so)
 	dn.get("items")[0].qty = delivered_qty or 5
 	dn.insert()
-	dn.submit()
+	if not do_not_submit:
+		dn.submit()
 	return dn
 
 
diff --git a/erpnext/stock/doctype/delivery_note/delivery_note.py b/erpnext/stock/doctype/delivery_note/delivery_note.py
index a7fcd04c634b..4c2c023b6ae3 100644
--- a/erpnext/stock/doctype/delivery_note/delivery_note.py
+++ b/erpnext/stock/doctype/delivery_note/delivery_note.py
@@ -251,6 +251,7 @@ def so_required(self):
 	def validate(self):
 		self.validate_posting_time()
 		super(DeliveryNote, self).validate()
+		self.validate_references()
 		self.set_status()
 		self.so_required()
 		self.validate_proj_cust()
@@ -341,6 +342,58 @@ def set_serial_and_batch_bundle_from_pick_list(self):
 
 					item.serial_and_batch_bundle = cls_obj.serial_and_batch_bundle
 
+	def validate_references(self):
+		self.validate_sales_order_references()
+		self.validate_sales_invoice_references()
+
+	def validate_sales_order_references(self):
+		err_msg = ""
+		for item in self.items:
+			if (item.against_sales_order and not item.so_detail) or (
+				not item.against_sales_order and item.so_detail
+			):
+				if not item.against_sales_order:
+					err_msg += (
+						_("'Sales Order' reference ({1}) is missing in row {0}").format(
+							frappe.bold(item.idx), frappe.bold("against_sales_order")
+						)
+						+ "<br>"
+					)
+				else:
+					err_msg += (
+						_("'Sales Order Item' reference ({1}) is missing in row {0}").format(
+							frappe.bold(item.idx), frappe.bold("so_detail")
+						)
+						+ "<br>"
+					)
+
+		if err_msg:
+			frappe.throw(err_msg, title=_("References to Sales Orders are Incomplete"))
+
+	def validate_sales_invoice_references(self):
+		err_msg = ""
+		for item in self.items:
+			if (item.against_sales_invoice and not item.si_detail) or (
+				not item.against_sales_invoice and item.si_detail
+			):
+				if not item.against_sales_invoice:
+					err_msg += (
+						_("'Sales Invoice' reference ({1}) is missing in row {0}").format(
+							frappe.bold(item.idx), frappe.bold("against_sales_invoice")
+						)
+						+ "<br>"
+					)
+				else:
+					err_msg += (
+						_("'Sales Invoice Item' reference ({1}) is missing in row {0}").format(
+							frappe.bold(item.idx), frappe.bold("si_detail")
+						)
+						+ "<br>"
+					)
+
+		if err_msg:
+			frappe.throw(err_msg, title=_("References to Sales Invoices are Incomplete"))
+
 	def validate_proj_cust(self):
 		"""check for does customer belong to same project as entered.."""
 		if self.project and self.customer:
diff --git a/erpnext/stock/doctype/delivery_note/test_delivery_note.py b/erpnext/stock/doctype/delivery_note/test_delivery_note.py
index 808862949584..f9341393ad01 100644
--- a/erpnext/stock/doctype/delivery_note/test_delivery_note.py
+++ b/erpnext/stock/doctype/delivery_note/test_delivery_note.py
@@ -813,6 +813,15 @@ def test_closed_delivery_note(self):
 		dn.cancel()
 		self.assertEqual(dn.status, "Cancelled")
 
+	def test_sales_order_reference_validation(self):
+		so = make_sales_order(po_no="12345")
+		dn = create_dn_against_so(so.name, delivered_qty=2, do_not_submit=True)
+		dn.items[0].against_sales_order = None
+		self.assertRaises(frappe.ValidationError, dn.save)
+		dn.reload()
+		dn.items[0].so_detail = None
+		self.assertRaises(frappe.ValidationError, dn.save)
+
 	def test_dn_billing_status_case1(self):
 		# SO -> DN -> SI
 		so = make_sales_order(po_no="12345")