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ProtHint Usage Example

Description

This folder contains an example input which serves to:

  • Demonstrate the usage of ProtHint
  • To test whether the program is correctly configured

The example input genome comprises of the first 3 Mbp in Drosophila melanogaster's 2L chromosome.

Running the pipeline

To run ProtHint, use the following command

../bin/prothint.py input/genome.fasta input/proteins.fasta --geneSeeds input/genemark.gtf --workdir test

If everything is configured correctly, the results in the test folder should match the contents of the output folder. Note that the order of hints in gff files might differ.

To check, use for example:

diff <(sort test/prothint.gff) <(sort output/prothint.gff)
diff <(sort test/evidence.gff) <(sort output/evidence.gff)

The expected runtime for this example on a system with 8 CPUs (each 2.40GHz) and 8GB of RAM is about 1.5 minutes.