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Suggestion: cell area report in the output file #49

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alfonsecard opened this issue Apr 12, 2022 · 2 comments
Open

Suggestion: cell area report in the output file #49

alfonsecard opened this issue Apr 12, 2022 · 2 comments
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enhancement New feature or request

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@alfonsecard
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Hi,

First of all thanks a lot for sharing your work, I've been using the MATLAB version of your software for some years, and now I'm trying to move to your new version in ImJoy.

Since sometimes is quite complicated to conclude about gene expression levels (considering that the number of transcripts is also dependent on cell size), I've been using the #transcripts/cell area as a value to evaluate gene expression. I realized that you removed the cell area calculation from the final output file (that could be found in the MATLAB output before), so I would like to know if you have any plugin that can help to assign a number of area pixels to each segmented cell.

Thanks again!

@muellerflorian muellerflorian added the enhancement New feature or request label Apr 14, 2022
@muellerflorian
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Hello,

thanks for bringing this up. You are right, currently, we only report expression levels per cell.

@Henley13 , what about reporting cell and nuclear size in addition to expression levels? This could either be by default or an option. essentially, this concerns multistack.summarize_extraction_results

@Henley13
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Hi,

The area is available in the new version (0.6.2). It's done by default. We should update the ImJoy plugin now...

Best,
Arthur

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