diff --git a/index.js b/index.js
index 47cfee7e..5309e27b 100644
--- a/index.js
+++ b/index.js
@@ -26,11 +26,6 @@ const fastifyAutoload = async function autoload (fastify, options) {
   const pluginArray = [].concat.apply([], Object.values(pluginTree).map(o => o.plugins))
   const hookArray = [].concat.apply([], Object.values(pluginTree).map(o => o.hooks))
 
-  let rootPrefix = options.options?.prefix ?? ''
-  // when prefix is provided /prefix/ format
-  // it is not good for concat, since most folder prefix start with /
-  if (rootPrefix[rootPrefix.length - 1] === '/') rootPrefix = rootPrefix.slice(0, -1)
-
   await Promise.all(pluginArray.map(({ file, type, prefix }) => {
     return loadPlugin({ file, type, directoryPrefix: prefix, options: opts, log: fastify.log })
       .then((plugin) => {
@@ -90,7 +85,7 @@ const fastifyAutoload = async function autoload (fastify, options) {
       }
 
       fastify.register(composedPlugin, {
-        prefix: rootPrefix + replaceRouteParamPattern(prefix)
+        prefix: options.options?.prefix ?? replaceRouteParamPattern(prefix)
       })
     }
   }
diff --git a/test/issues/326/test.js b/test/issues/326/test.js
index ba8ab23a..58f4f99f 100644
--- a/test/issues/326/test.js
+++ b/test/issues/326/test.js
@@ -62,7 +62,7 @@ app.ready(function (err) {
   })
 
   app.inject({
-    url: '/custom-prefix/child'
+    url: '/custom-prefix'
   }, function (err, res) {
     t.error(err)
 
@@ -70,7 +70,7 @@ app.ready(function (err) {
   })
 
   app.inject({
-    url: '/custom-prefix/child/not-exists'
+    url: '/custom-prefix/not-exists'
   }, function (err, res) {
     t.error(err)
     t.equal(res.headers.from, 'routes-b')
diff --git a/test/issues/376/routes/entity/.autohooks.js b/test/issues/376/routes/entity/.autohooks.js
deleted file mode 100644
index 951e9583..00000000
--- a/test/issues/376/routes/entity/.autohooks.js
+++ /dev/null
@@ -1,8 +0,0 @@
-'use strict'
-
-module.exports = async function (app, opts) {
-  app.addHook('onRequest', async (req, reply) => {
-      req.hooked = req.hooked || []
-      req.hooked.push('root')
-    })
-}
diff --git a/test/issues/376/routes/entity/index.js b/test/issues/376/routes/entity/index.js
deleted file mode 100644
index 0b14399b..00000000
--- a/test/issues/376/routes/entity/index.js
+++ /dev/null
@@ -1,7 +0,0 @@
-'use strict'
-
-module.exports = async function (app, opts, next) {
-  app.get('/', async function (req, reply) {
-    reply.status(200).send({ path: req.url, hooked: req.hooked })
-  })
-}
diff --git a/test/issues/376/routes/index.js b/test/issues/376/routes/index.js
deleted file mode 100644
index 51a699b4..00000000
--- a/test/issues/376/routes/index.js
+++ /dev/null
@@ -1,7 +0,0 @@
-'use strict'
-
-module.exports = async function (app, opts, next) {
-  app.get('/', async function (req, reply) {
-    reply.status(200).send({ path: req.url })
-  })
-}
diff --git a/test/issues/376/test.js b/test/issues/376/test.js
deleted file mode 100644
index d1f42b2d..00000000
--- a/test/issues/376/test.js
+++ /dev/null
@@ -1,58 +0,0 @@
-'use strict'
-
-const t = require('tap')
-const path = require('node:path')
-const Fastify = require('fastify')
-const autoLoad = require('../../../')
-
-t.plan(13)
-
-const app = Fastify()
-
-app.register(autoLoad, {
-  dir: path.join(__dirname, 'routes'),
-  autoHooks: true,
-  options: { prefix: '/api' }
-})
-
-app.register(autoLoad, {
-  dir: path.join(__dirname, 'routes'),
-  autoHooks: true,
-  options: { prefix: '/prefix/' }
-})
-
-app.ready(function (err) {
-  t.error(err)
-
-  app.inject({
-    url: '/api'
-  }, function (err, res) {
-    t.error(err)
-    t.equal(res.statusCode, 200)
-    t.same(res.json(), { path: '/api' })
-  })
-
-  app.inject({
-    url: '/api/entity'
-  }, function (err, res) {
-    t.error(err)
-    t.equal(res.statusCode, 200)
-    t.same(res.json(), { path: '/api/entity', hooked: ['root'] })
-  })
-
-  app.inject({
-    url: '/prefix'
-  }, function (err, res) {
-    t.error(err)
-    t.equal(res.statusCode, 200)
-    t.same(res.json(), { path: '/prefix' })
-  })
-
-  app.inject({
-    url: '/prefix/entity'
-  }, function (err, res) {
-    t.error(err)
-    t.equal(res.statusCode, 200)
-    t.same(res.json(), { path: '/prefix/entity', hooked: ['root'] })
-  })
-})