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FastQC Disable Overrepresented sequences #1488

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schorlton opened this issue Jul 9, 2021 · 1 comment · Fixed by #1497
Closed

FastQC Disable Overrepresented sequences #1488

schorlton opened this issue Jul 9, 2021 · 1 comment · Fixed by #1497
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bug: core Bug in the main MultiQC code

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@schorlton
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Description of bug

I filed a previous bug regarding MultiQC failing when I disabled overrepresented sequences in the FastQC config, and some work went into fixing it: #1281

I tried it out on a new file but unfortunately I'm hitting another error. Example file and log attached. Thanks for your hard work!!

File that triggers the error

test_fastqc.zip

MultiQC Error log

/// MultiQC 🔍 | v1.11

|           multiqc | Search path : /test/test_fastqc.zip
|         searching | ━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━ 100% 1/1  
|            fastqc | Found 1 reports
╭──────────────────── Oops! The 'fastqc' MultiQC module broke... ────────────────────╮
│ Please copy this log and report it at https://github.com/ewels/MultiQC/issues      │
│ Please attach a file that triggers the error. The last file found was:             │
│ test_fastqc.zip                                                                    │
│                                                                                    │
│ Traceback (most recent call last):                                                 │
│   File "/opt/conda/envs/bugseq/lib/python3.9/site-packages/multiqc/multiqc.py", li │
│     output = mod()                                                                 │
│   File "/opt/conda/envs/bugseq/lib/python3.9/site-packages/multiqc/modules/fastqc/ │
│     self.overrepresented_sequences()                                               │
│   File "/opt/conda/envs/bugseq/lib/python3.9/site-packages/multiqc/modules/fastqc/ │
│     if all(len(data[s_name]) == 0 for s_name in self.fastqc_data):                 │
│   File "/opt/conda/envs/bugseq/lib/python3.9/site-packages/multiqc/modules/fastqc/ │
│     if all(len(data[s_name]) == 0 for s_name in self.fastqc_data):                 │
│ KeyError: 'f759aea1_barcode23'                                                     │
│                                                                                    │
╰────────────────────────────────────────────────────────────────────────────────────╯
|           multiqc | No analysis results found. Cleaning up..
|           multiqc | MultiQC complete
@ewels ewels added the bug: core Bug in the main MultiQC code label Jul 10, 2021
ewels added a commit to MultiQC/test-data that referenced this issue Nov 7, 2021
ewels added a commit to MultiQC/test-data that referenced this issue Nov 7, 2021
@ewels
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ewels commented Nov 7, 2021

Thanks for reporting this (with the test data) @schorlton! Fixed by @ErikDanielsson in #1497 🚀

vladsavelyev pushed a commit to vladsavelyev/MultiQC_TestData that referenced this issue Apr 16, 2022
vladsavelyev pushed a commit to vladsavelyev/MultiQC_TestData that referenced this issue Apr 16, 2022
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Labels
bug: core Bug in the main MultiQC code
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