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Description of bug:
PCT_USABLE_BASES_ON_BAIT and PCT_USABLE_BASES_ON_TARGET columns in Picard HsMetrics are being processed as bases rather than percentages by MultiQC.
MultiQC run details (please complete the following):
Command used to run MultiQC: multiqc .
MultiQC Version: MultiQC v1.9
Operating System: Red Hat Enterprise Linux 7.5
Python Version: Python 3.8.1
Method of MultiQC installation: manual
Additional context
The issue is understandable, since Picard descriptions on these columns are:
"The number of aligned, de-duped, on-bait bases out of the PF bases available."
"The number of aligned, de-duped, on-target bases out of the PF bases available."
although these columns report percentages (values from 0 to 1) rather than number of bases (as one may deduct from the PCT_ column name). https://broadinstitute.github.io/picard/javadoc/picard/picard/analysis/directed/HsMetrics.html
What works from me
I've edited HsMetrics.py module as follows, in case you want to consider something similar. I've included a few lines after line 277 to detect those columns and manually change their content:
Description of bug:
PCT_USABLE_BASES_ON_BAIT and PCT_USABLE_BASES_ON_TARGET columns in Picard HsMetrics are being processed as bases rather than percentages by MultiQC.
MultiQC run details (please complete the following):
Additional context
The issue is understandable, since Picard descriptions on these columns are:
"The number of aligned, de-duped, on-bait bases out of the PF bases available."
"The number of aligned, de-duped, on-target bases out of the PF bases available."
although these columns report percentages (values from 0 to 1) rather than number of bases (as one may deduct from the PCT_ column name).
https://broadinstitute.github.io/picard/javadoc/picard/picard/analysis/directed/HsMetrics.html
What works from me
I've edited HsMetrics.py module as follows, in case you want to consider something similar. I've included a few lines after line 277 to detect those columns and manually change their content:
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