diff --git a/samples/bluetooth/hci_ipc/nrf5340_cpunet_bis-bt_ll_sw_split.conf b/samples/bluetooth/hci_ipc/nrf5340_cpunet_bis-bt_ll_sw_split.conf
index a7487118694..13b4268e165 100644
--- a/samples/bluetooth/hci_ipc/nrf5340_cpunet_bis-bt_ll_sw_split.conf
+++ b/samples/bluetooth/hci_ipc/nrf5340_cpunet_bis-bt_ll_sw_split.conf
@@ -5,7 +5,7 @@ CONFIG_ISR_STACK_SIZE=1024
 CONFIG_IDLE_STACK_SIZE=256
 CONFIG_MAIN_STACK_SIZE=512
 CONFIG_SYSTEM_WORKQUEUE_STACK_SIZE=512
-CONFIG_HEAP_MEM_POOL_SIZE=8192
+CONFIG_HEAP_MEM_POOL_SIZE=6144
 
 CONFIG_BT=y
 CONFIG_BT_HCI_RAW=y
@@ -40,7 +40,11 @@ CONFIG_BT_ISO_PERIPHERAL=n
 
 # ISO Streams
 CONFIG_BT_ISO_MAX_CHAN=4
-CONFIG_BT_ISO_TX_BUF_COUNT=1
+# In theory, CONFIG_BT_ISO_TX_BUF_COUNT=1, should be sufficient but this count
+# is used in the context of IPC which falls into a "Newton's Cradle" effect
+# where probably (CONFIG_BT_CTLR_ISO_TX_BUFFERS - CONFIG_BT_ISO_TX_BUF_COUNT)
+# buffers get throttled. Hence, always have the value equal or greater.
+CONFIG_BT_ISO_TX_BUF_COUNT=12
 CONFIG_BT_ISO_RX_BUF_COUNT=1
 
 # Controller
diff --git a/samples/bluetooth/hci_ipc/nrf5340_cpunet_bt_mesh-bt_ll_sw_split.conf b/samples/bluetooth/hci_ipc/nrf5340_cpunet_bt_mesh-bt_ll_sw_split.conf
index 0d78cf862ee..8cc6ac936c9 100644
--- a/samples/bluetooth/hci_ipc/nrf5340_cpunet_bt_mesh-bt_ll_sw_split.conf
+++ b/samples/bluetooth/hci_ipc/nrf5340_cpunet_bt_mesh-bt_ll_sw_split.conf
@@ -1,7 +1,7 @@
 CONFIG_IPC_SERVICE=y
 CONFIG_MBOX=y
 
-CONFIG_HEAP_MEM_POOL_SIZE=8192
+CONFIG_HEAP_MEM_POOL_SIZE=6144
 
 CONFIG_MAIN_STACK_SIZE=512
 CONFIG_SYSTEM_WORKQUEUE_STACK_SIZE=512
diff --git a/samples/bluetooth/hci_ipc/nrf5340_cpunet_cis-bt_ll_sw_split.conf b/samples/bluetooth/hci_ipc/nrf5340_cpunet_cis-bt_ll_sw_split.conf
index f7e1f8fc673..19b78f03302 100644
--- a/samples/bluetooth/hci_ipc/nrf5340_cpunet_cis-bt_ll_sw_split.conf
+++ b/samples/bluetooth/hci_ipc/nrf5340_cpunet_cis-bt_ll_sw_split.conf
@@ -5,7 +5,7 @@ CONFIG_ISR_STACK_SIZE=1024
 CONFIG_IDLE_STACK_SIZE=256
 CONFIG_MAIN_STACK_SIZE=512
 CONFIG_SYSTEM_WORKQUEUE_STACK_SIZE=512
-CONFIG_HEAP_MEM_POOL_SIZE=8192
+CONFIG_HEAP_MEM_POOL_SIZE=6144
 
 CONFIG_BT=y
 CONFIG_BT_HCI_RAW=y
@@ -39,7 +39,11 @@ CONFIG_BT_ISO_PERIPHERAL=y
 
 # ISO Streams
 CONFIG_BT_ISO_MAX_CHAN=4
-CONFIG_BT_ISO_TX_BUF_COUNT=1
+# In theory, CONFIG_BT_ISO_TX_BUF_COUNT=1, should be sufficient but this count
+# is used in the context of IPC which falls into a "Newton's Cradle" effect
+# where probably (CONFIG_BT_CTLR_ISO_TX_BUFFERS - CONFIG_BT_ISO_TX_BUF_COUNT)
+# buffers get throttled. Hence, always have the value equal or greater.
+CONFIG_BT_ISO_TX_BUF_COUNT=12
 CONFIG_BT_ISO_RX_BUF_COUNT=1
 
 # Controller
@@ -82,8 +86,8 @@ CONFIG_BT_CTLR_LLCP_LOCAL_PROC_CTX_BUF_NUM=6
 # ISO Connection Oriented
 CONFIG_BT_CTLR_CENTRAL_ISO=y
 CONFIG_BT_CTLR_PERIPHERAL_ISO=y
-CONFIG_BT_CTLR_CONN_ISO_STREAMS=2
 CONFIG_BT_CTLR_CONN_ISO_GROUPS=1
+CONFIG_BT_CTLR_CONN_ISO_STREAMS=2
 CONFIG_BT_CTLR_CONN_ISO_STREAMS_PER_GROUP=2
 CONFIG_BT_CTLR_CONN_ISO_SDU_LEN_MAX=247
 CONFIG_BT_CTLR_CONN_ISO_PDU_LEN_MAX=251
diff --git a/samples/bluetooth/hci_ipc/nrf5340_cpunet_df-bt_ll_sw_split.conf b/samples/bluetooth/hci_ipc/nrf5340_cpunet_df-bt_ll_sw_split.conf
index b183a4c9825..3859fc011f8 100644
--- a/samples/bluetooth/hci_ipc/nrf5340_cpunet_df-bt_ll_sw_split.conf
+++ b/samples/bluetooth/hci_ipc/nrf5340_cpunet_df-bt_ll_sw_split.conf
@@ -5,7 +5,7 @@ CONFIG_ISR_STACK_SIZE=1024
 CONFIG_IDLE_STACK_SIZE=256
 CONFIG_MAIN_STACK_SIZE=512
 CONFIG_SYSTEM_WORKQUEUE_STACK_SIZE=512
-CONFIG_HEAP_MEM_POOL_SIZE=8192
+CONFIG_HEAP_MEM_POOL_SIZE=6144
 
 CONFIG_BT=y
 CONFIG_BT_HCI_RAW=y
diff --git a/samples/bluetooth/hci_ipc/nrf5340_cpunet_iso-bt_ll_sw_split.conf b/samples/bluetooth/hci_ipc/nrf5340_cpunet_iso-bt_ll_sw_split.conf
index 925eac8e34e..5f10718be24 100644
--- a/samples/bluetooth/hci_ipc/nrf5340_cpunet_iso-bt_ll_sw_split.conf
+++ b/samples/bluetooth/hci_ipc/nrf5340_cpunet_iso-bt_ll_sw_split.conf
@@ -5,7 +5,7 @@ CONFIG_ISR_STACK_SIZE=1024
 CONFIG_IDLE_STACK_SIZE=256
 CONFIG_MAIN_STACK_SIZE=512
 CONFIG_SYSTEM_WORKQUEUE_STACK_SIZE=512
-CONFIG_HEAP_MEM_POOL_SIZE=8192
+CONFIG_HEAP_MEM_POOL_SIZE=6144
 CONFIG_CBPRINTF_REDUCED_INTEGRAL=y
 
 CONFIG_ISR_TABLES_LOCAL_DECLARATION=y
@@ -46,7 +46,11 @@ CONFIG_BT_ISO_PERIPHERAL=y
 CONFIG_BT_ISO_TX_MTU=310
 CONFIG_BT_ISO_RX_MTU=310
 CONFIG_BT_ISO_MAX_CHAN=4
-CONFIG_BT_ISO_TX_BUF_COUNT=1
+# In theory, CONFIG_BT_ISO_TX_BUF_COUNT=1, should be sufficient but this count
+# is used in the context of IPC which falls into a "Newton's Cradle" effect
+# where probably (CONFIG_BT_CTLR_ISO_TX_BUFFERS - CONFIG_BT_ISO_TX_BUF_COUNT)
+# buffers get throttled. Hence, always have the value equal or greater.
+CONFIG_BT_ISO_TX_BUF_COUNT=8
 CONFIG_BT_ISO_RX_BUF_COUNT=1
 
 # Controller
@@ -100,8 +104,8 @@ CONFIG_BT_CTLR_ADV_EXT=y
 CONFIG_BT_CTLR_ADV_PERIODIC=y
 CONFIG_BT_CTLR_SYNC_TRANSFER_SENDER=y
 CONFIG_BT_CTLR_ADV_ISO=y
-CONFIG_BT_CTLR_ADV_ISO_SET=2
-CONFIG_BT_CTLR_ADV_ISO_STREAM_COUNT=4
+CONFIG_BT_CTLR_ADV_ISO_SET=1
+CONFIG_BT_CTLR_ADV_ISO_STREAM_COUNT=2
 CONFIG_BT_CTLR_ADV_ISO_STREAM_MAX=2
 CONFIG_BT_CTLR_ADV_ISO_PDU_LEN_MAX=247
 
@@ -118,16 +122,16 @@ CONFIG_BT_CTLR_SYNC_ISO_PDU_LEN_MAX=251
 # ISO Connection Oriented
 CONFIG_BT_CTLR_CENTRAL_ISO=y
 CONFIG_BT_CTLR_PERIPHERAL_ISO=y
-CONFIG_BT_CTLR_CONN_ISO_STREAMS=2
 CONFIG_BT_CTLR_CONN_ISO_GROUPS=1
+CONFIG_BT_CTLR_CONN_ISO_STREAMS=2
 CONFIG_BT_CTLR_CONN_ISO_STREAMS_PER_GROUP=2
 CONFIG_BT_CTLR_CONN_ISO_SDU_LEN_MAX=247
 CONFIG_BT_CTLR_CONN_ISO_PDU_LEN_MAX=251
 CONFIG_BT_CTLR_CONN_ISO_LOW_LATENCY_POLICY=y
 
 # ISO Transmissions
-CONFIG_BT_CTLR_ISOAL_SOURCES=4
-CONFIG_BT_CTLR_ISO_TX_BUFFERS=12
+CONFIG_BT_CTLR_ISOAL_SOURCES=2
+CONFIG_BT_CTLR_ISO_TX_BUFFERS=8
 CONFIG_BT_CTLR_ISO_TX_BUFFER_SIZE=255
 
 # ISO Receptions
diff --git a/samples/bluetooth/hci_ipc/nrf5340_cpunet_iso_broadcast-bt_ll_sw_split.conf b/samples/bluetooth/hci_ipc/nrf5340_cpunet_iso_broadcast-bt_ll_sw_split.conf
index 67c5854c9c0..8bcce089c6e 100644
--- a/samples/bluetooth/hci_ipc/nrf5340_cpunet_iso_broadcast-bt_ll_sw_split.conf
+++ b/samples/bluetooth/hci_ipc/nrf5340_cpunet_iso_broadcast-bt_ll_sw_split.conf
@@ -5,7 +5,7 @@ CONFIG_ISR_STACK_SIZE=1024
 CONFIG_IDLE_STACK_SIZE=256
 CONFIG_MAIN_STACK_SIZE=512
 CONFIG_SYSTEM_WORKQUEUE_STACK_SIZE=512
-CONFIG_HEAP_MEM_POOL_SIZE=8192
+CONFIG_HEAP_MEM_POOL_SIZE=6144
 
 CONFIG_BT=y
 CONFIG_BT_HCI_RAW=y
@@ -20,11 +20,18 @@ CONFIG_BT_OBSERVER=n
 CONFIG_BT_EXT_ADV=y
 CONFIG_BT_PER_ADV=y
 CONFIG_BT_ISO_BROADCASTER=y
-CONFIG_BT_ISO_MAX_CHAN=4
-CONFIG_BT_ISO_TX_BUF_COUNT=1
 CONFIG_BT_CENTRAL=n
 CONFIG_BT_PERIPHERAL=n
 
+# ISO Streams
+CONFIG_BT_ISO_MAX_CHAN=4
+# In theory, CONFIG_BT_ISO_TX_BUF_COUNT=1, should be sufficient but this count
+# is used in the context of IPC which falls into a "Newton's Cradle" effect
+# where probably (CONFIG_BT_CTLR_ISO_TX_BUFFERS - CONFIG_BT_ISO_TX_BUF_COUNT)
+# buffers get throttled. Hence, always have the value equal or greater.
+CONFIG_BT_ISO_TX_BUF_COUNT=12
+CONFIG_BT_ISO_RX_BUF_COUNT=1
+
 # ISO Broadcast Controller
 CONFIG_BT_LL_SW_SPLIT=y
 CONFIG_BT_CTLR_ADV_PERIODIC=y
diff --git a/samples/bluetooth/hci_ipc/nrf5340_cpunet_iso_central-bt_ll_sw_split.conf b/samples/bluetooth/hci_ipc/nrf5340_cpunet_iso_central-bt_ll_sw_split.conf
index 505b9d352ba..2a450dfdf2e 100644
--- a/samples/bluetooth/hci_ipc/nrf5340_cpunet_iso_central-bt_ll_sw_split.conf
+++ b/samples/bluetooth/hci_ipc/nrf5340_cpunet_iso_central-bt_ll_sw_split.conf
@@ -5,7 +5,7 @@ CONFIG_ISR_STACK_SIZE=1024
 CONFIG_IDLE_STACK_SIZE=256
 CONFIG_MAIN_STACK_SIZE=512
 CONFIG_SYSTEM_WORKQUEUE_STACK_SIZE=512
-CONFIG_HEAP_MEM_POOL_SIZE=8192
+CONFIG_HEAP_MEM_POOL_SIZE=6144
 
 CONFIG_BT=y
 CONFIG_BT_HCI_RAW=y
@@ -35,7 +35,11 @@ CONFIG_BT_ISO_PERIPHERAL=n
 
 # ISO Streams
 CONFIG_BT_ISO_MAX_CHAN=2
-CONFIG_BT_ISO_TX_BUF_COUNT=1
+# In theory, CONFIG_BT_ISO_TX_BUF_COUNT=1, should be sufficient but this count
+# is used in the context of IPC which falls into a "Newton's Cradle" effect
+# where probably (CONFIG_BT_CTLR_ISO_TX_BUFFERS - CONFIG_BT_ISO_TX_BUF_COUNT)
+# buffers get throttled. Hence, always have the value equal or greater.
+CONFIG_BT_ISO_TX_BUF_COUNT=12
 CONFIG_BT_ISO_RX_BUF_COUNT=1
 
 # Controller
@@ -46,8 +50,8 @@ CONFIG_BT_CTLR_SCAN_DATA_LEN_MAX=191
 # ISO Connection Oriented
 CONFIG_BT_CTLR_CENTRAL_ISO=y
 CONFIG_BT_CTLR_PERIPHERAL_ISO=n
-CONFIG_BT_CTLR_CONN_ISO_STREAMS=2
 CONFIG_BT_CTLR_CONN_ISO_GROUPS=1
+CONFIG_BT_CTLR_CONN_ISO_STREAMS=2
 CONFIG_BT_CTLR_CONN_ISO_STREAMS_PER_GROUP=2
 CONFIG_BT_CTLR_CONN_ISO_SDU_LEN_MAX=247
 CONFIG_BT_CTLR_CONN_ISO_PDU_LEN_MAX=251
diff --git a/samples/bluetooth/hci_ipc/nrf5340_cpunet_iso_peripheral-bt_ll_sw_split.conf b/samples/bluetooth/hci_ipc/nrf5340_cpunet_iso_peripheral-bt_ll_sw_split.conf
index 05c9a9d3903..31eb9f6669e 100644
--- a/samples/bluetooth/hci_ipc/nrf5340_cpunet_iso_peripheral-bt_ll_sw_split.conf
+++ b/samples/bluetooth/hci_ipc/nrf5340_cpunet_iso_peripheral-bt_ll_sw_split.conf
@@ -5,7 +5,7 @@ CONFIG_ISR_STACK_SIZE=1024
 CONFIG_IDLE_STACK_SIZE=256
 CONFIG_MAIN_STACK_SIZE=512
 CONFIG_SYSTEM_WORKQUEUE_STACK_SIZE=512
-CONFIG_HEAP_MEM_POOL_SIZE=8192
+CONFIG_HEAP_MEM_POOL_SIZE=6144
 
 CONFIG_BT=y
 CONFIG_BT_HCI_RAW=y
@@ -35,7 +35,11 @@ CONFIG_BT_ISO_PERIPHERAL=y
 
 # ISO Streams
 CONFIG_BT_ISO_MAX_CHAN=2
-CONFIG_BT_ISO_TX_BUF_COUNT=1
+# In theory, CONFIG_BT_ISO_TX_BUF_COUNT=1, should be sufficient but this count
+# is used in the context of IPC which falls into a "Newton's Cradle" effect
+# where probably (CONFIG_BT_CTLR_ISO_TX_BUFFERS - CONFIG_BT_ISO_TX_BUF_COUNT)
+# buffers get throttled. Hence, always have the value equal or greater.
+CONFIG_BT_ISO_TX_BUF_COUNT=12
 CONFIG_BT_ISO_RX_BUF_COUNT=1
 
 # Controller
@@ -46,8 +50,8 @@ CONFIG_BT_CTLR_ADV_DATA_LEN_MAX=191
 # ISO Connection Oriented
 CONFIG_BT_CTLR_CENTRAL_ISO=n
 CONFIG_BT_CTLR_PERIPHERAL_ISO=y
-CONFIG_BT_CTLR_CONN_ISO_STREAMS=2
 CONFIG_BT_CTLR_CONN_ISO_GROUPS=1
+CONFIG_BT_CTLR_CONN_ISO_STREAMS=2
 CONFIG_BT_CTLR_CONN_ISO_STREAMS_PER_GROUP=2
 CONFIG_BT_CTLR_CONN_ISO_SDU_LEN_MAX=247
 CONFIG_BT_CTLR_CONN_ISO_PDU_LEN_MAX=251
diff --git a/samples/bluetooth/hci_ipc/nrf5340_cpunet_iso_receive-bt_ll_sw_split.conf b/samples/bluetooth/hci_ipc/nrf5340_cpunet_iso_receive-bt_ll_sw_split.conf
index 51ca53e83f2..872da8f681b 100644
--- a/samples/bluetooth/hci_ipc/nrf5340_cpunet_iso_receive-bt_ll_sw_split.conf
+++ b/samples/bluetooth/hci_ipc/nrf5340_cpunet_iso_receive-bt_ll_sw_split.conf
@@ -5,7 +5,7 @@ CONFIG_ISR_STACK_SIZE=1024
 CONFIG_IDLE_STACK_SIZE=256
 CONFIG_MAIN_STACK_SIZE=512
 CONFIG_SYSTEM_WORKQUEUE_STACK_SIZE=512
-CONFIG_HEAP_MEM_POOL_SIZE=8192
+CONFIG_HEAP_MEM_POOL_SIZE=6144
 
 CONFIG_BT=y
 CONFIG_BT_HCI_RAW=y
diff --git a/samples/bluetooth/hci_ipc/overlay-nrf5340_cpunet_iso_nrf21540_ek-bt_ll_sw_split.conf b/samples/bluetooth/hci_ipc/overlay-nrf5340_cpunet_iso_nrf21540_ek-bt_ll_sw_split.conf
index daea503621a..12ab4e53eb9 100644
--- a/samples/bluetooth/hci_ipc/overlay-nrf5340_cpunet_iso_nrf21540_ek-bt_ll_sw_split.conf
+++ b/samples/bluetooth/hci_ipc/overlay-nrf5340_cpunet_iso_nrf21540_ek-bt_ll_sw_split.conf
@@ -1,4 +1,6 @@
 # Reduce from 310 bytes, in nrf5340_cpunet_iso-bt_ll_sw_split.conf
-# to be able to fit in 64KB RAM.
-CONFIG_BT_ISO_TX_MTU=247
-CONFIG_BT_ISO_RX_MTU=251
+# to be able to fit in 64KB RAM, in case needed in the future.
+
+# Example:
+# CONFIG_BT_ISO_TX_MTU=247
+# CONFIG_BT_ISO_RX_MTU=251