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How to merge BMMC and HSPC #2

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danielee0707 opened this issue Mar 31, 2024 · 1 comment
Open

How to merge BMMC and HSPC #2

danielee0707 opened this issue Mar 31, 2024 · 1 comment

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@danielee0707
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danielee0707 commented Mar 31, 2024

Hi, thank you for sharing the reproducibility notebook! May I ask how the RDS at /data/Multiome/DN4_BMMC_HSPC_Multiome_wrapper.filtered.anno in Note-4 was generated from individual datasets? I may have missed this part in the notebooks, and if so please point me to the right one. The Get_Started notebook only shows steps for one dataset. How did you merge the processed results for HSPC with BMMC, or were they concatenated first before running RedeemR? I also noticed that the number of cells in the provided Seurat multi-omic annotated object is slightly different from the sum of the RedeemR outputs for the two datasets, so was one further filtered towards the other one?

@chenweng1991
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Thank you @danielee0707, please refer to chenweng1991/redeemR#5
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Chen

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