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The Cell barcode not matched between Seurat object and redeemR object is expected, because:
the Seurat object derived from CellRanger output report RNA barcode, while the redeem reports the ATAC barcode. There is an easy way to translate the barcode from both sides. I provided a function Translate_simple_RNA2ATAC and Translate_simple_ATAC2RNA in the package redeemR. These two function input a vector of cell barcode and from one and translate to the other. The source information is here 10X document The RNA and ATAC barcodes are line-by-line corresponding to each other in the two files provided.
The text was updated successfully, but these errors were encountered:
The Cell barcode not matched between Seurat object and redeemR object is expected, because:
the Seurat object derived from CellRanger output report RNA barcode, while the redeem reports the ATAC barcode. There is an easy way to translate the barcode from both sides. I provided a function Translate_simple_RNA2ATAC and Translate_simple_ATAC2RNA in the package redeemR. These two function input a vector of cell barcode and from one and translate to the other. The source information is here 10X document The RNA and ATAC barcodes are line-by-line corresponding to each other in the two files provided.
The text was updated successfully, but these errors were encountered: