diff --git a/packages/aws-cdk-lib/aws-ec2/lib/vpc-endpoint.ts b/packages/aws-cdk-lib/aws-ec2/lib/vpc-endpoint.ts
index d186d00987317..7e3cedf219e09 100644
--- a/packages/aws-cdk-lib/aws-ec2/lib/vpc-endpoint.ts
+++ b/packages/aws-cdk-lib/aws-ec2/lib/vpc-endpoint.ts
@@ -9,6 +9,7 @@ import { ISubnet, IVpc, SubnetSelection } from './vpc';
 import * as iam from '../../aws-iam';
 import * as cxschema from '../../cloud-assembly-schema';
 import { Aws, ContextProvider, IResource, Lazy, Resource, Stack, Token } from '../../core';
+import { PARTITION_MAP } from '../../region-info/build-tools/fact-tables';
 
 /**
  * A VPC endpoint.
@@ -666,8 +667,21 @@ export class InterfaceVpcEndpointAwsService implements IInterfaceVpcEndpointServ
         'redshift', 'redshift-data', 's3', 'sagemaker.api', 'sagemaker.featurestore-runtime', 'sagemaker.runtime', 'securityhub',
         'servicecatalog', 'sms', 'sqs', 'states', 'sts', 'sync-states', 'synthetics', 'transcribe', 'transcribestreaming', 'transfer',
         'workspaces', 'xray'],
+      'us-isof-': ['ecr.api', 'ecr.dkr'],
+      'eu-isoe-': ['ecr.api', 'ecr.dkr'],
     };
-    if (VPC_ENDPOINT_SERVICE_EXCEPTIONS[region]?.includes(name)) {
+
+    const regionPartition = region.split('-').slice(0, 2).join('-');
+    const partitionDetails = PARTITION_MAP[`${regionPartition}-`];
+
+    // Check for specific service name under isolated region prefix
+    const serviceInExceptions = VPC_ENDPOINT_SERVICE_EXCEPTIONS[`${regionPartition}-`]?.includes(name);
+
+    if (serviceInExceptions) {
+      // Endpoints generated in reverse of domain suffix for the services mentioned in map
+      const reverseString = partitionDetails.domainSuffix.split('.').reverse().join('.');
+      return reverseString;
+    } else if (VPC_ENDPOINT_SERVICE_EXCEPTIONS[region]?.includes(name)) {
       return 'cn.com.amazonaws';
     } else {
       return 'com.amazonaws';
diff --git a/packages/aws-cdk-lib/aws-ec2/test/vpc-endpoint.test.ts b/packages/aws-cdk-lib/aws-ec2/test/vpc-endpoint.test.ts
index 3c1c05ba8dfa8..2e9ae880d7147 100644
--- a/packages/aws-cdk-lib/aws-ec2/test/vpc-endpoint.test.ts
+++ b/packages/aws-cdk-lib/aws-ec2/test/vpc-endpoint.test.ts
@@ -694,6 +694,37 @@ describe('vpc endpoint', () => {
       });
 
     });
+
+    test.each([
+      ['us-isof-test-1', 'gov.ic.hci.csp'],
+      ['eu-isoe-test-1', 'uk.adc-e.cloud'],
+      ['us-east-1', 'com.amazonaws'],
+      ['us-gov-west-1', 'com.amazonaws'],
+      ['cn-northwest-1', 'cn.com.amazonaws'],
+      ['cn-north-1', 'cn.com.amazonaws'],
+    ])('test vpc interface endpoint for ECR can be created correctly in all regions', (region : string, domain: string) => {
+      //GIVEN
+      const stack = new Stack(undefined, 'TestStack', { env: { account: '123456789012', region: region } });
+      const vpc = new Vpc(stack, 'VPC');
+
+      //WHEN
+      vpc.addInterfaceEndpoint('ECR Endpoint', {
+        service: InterfaceVpcEndpointAwsService.ECR,
+      });
+
+      vpc.addInterfaceEndpoint('ECR Docker Endpoint', {
+        service: InterfaceVpcEndpointAwsService.ECR_DOCKER,
+      });
+
+      //THEN
+      Template.fromStack(stack).hasResourceProperties('AWS::EC2::VPCEndpoint', {
+        ServiceName: `${domain}.${region}.ecr.api`,
+      });
+      Template.fromStack(stack).hasResourceProperties('AWS::EC2::VPCEndpoint', {
+        ServiceName: `${domain}.${region}.ecr.dkr`,
+      });
+    });
+
     test.each([
       ['transcribe', InterfaceVpcEndpointAwsService.TRANSCRIBE],
     ])('test vpc interface endpoint with .cn suffix for %s can be created correctly in China regions', (name: string, given: InterfaceVpcEndpointAwsService) => {