diff --git a/dev/tasks/python-wheels/win-build.bat b/dev/tasks/python-wheels/win-build.bat
index 437c1408b60ee..b46c59dd709d5 100644
--- a/dev/tasks/python-wheels/win-build.bat
+++ b/dev/tasks/python-wheels/win-build.bat
@@ -27,7 +27,7 @@ conda create -n wheel-build -q -y -c conda-forge ^
     numpy=%NUMPY_VERSION% ^
     || exit /B
 
-conda activate wheel-build
+call conda.bat activate wheel-build
 
 @rem Cannot use conda_env_python.yml here because conda-forge has
 @rem ceased providing up-to-date packages for Python 3.5
@@ -84,7 +84,7 @@ pushd %ARROW_SRC%\python
 python setup.py bdist_wheel || exit /B
 popd
 
-conda deactivate
+call conda.bat deactivate
 
 set ARROW_TEST_DATA=%ARROW_SRC%\testing\data
 
@@ -94,7 +94,7 @@ conda create -n wheel-test -c conda-forge -q -y ^
     --file %ARROW_SRC%\ci\conda_env_python.yml ^
     python=%PYTHON_VERSION% ^
     numpy=%NUMPY_VERSION% || exit /B
-conda activate wheel-test
+call conda.bat activate wheel-test
 
 @rem install the built wheel
 pip install -vv --no-index --find-links=%ARROW_SRC%\python\dist\ pyarrow || exit /B