diff --git a/CHANGES/10102.bugfix.rst b/CHANGES/10102.bugfix.rst
new file mode 100644
index 00000000000..86dda8684dd
--- /dev/null
+++ b/CHANGES/10102.bugfix.rst
@@ -0,0 +1 @@
+Replaced deprecated call to :func:`mimetypes.guess_type` with :func:`mimetypes.guess_file_type` when using Python 3.13+ -- by :user:`bdraco`.
diff --git a/aiohttp/payload.py b/aiohttp/payload.py
index 1bc7f27de8b..55a7a677f49 100644
--- a/aiohttp/payload.py
+++ b/aiohttp/payload.py
@@ -4,6 +4,7 @@
 import json
 import mimetypes
 import os
+import sys
 import warnings
 from abc import ABC, abstractmethod
 from itertools import chain
@@ -169,7 +170,11 @@ def __init__(
             assert isinstance(content_type, str)
             self._headers[hdrs.CONTENT_TYPE] = content_type
         elif self._filename is not None:
-            content_type = mimetypes.guess_type(self._filename)[0]
+            if sys.version_info >= (3, 13):
+                guesser = mimetypes.guess_file_type
+            else:
+                guesser = mimetypes.guess_type
+            content_type = guesser(self._filename)[0]
             if content_type is None:
                 content_type = self._default_content_type
             self._headers[hdrs.CONTENT_TYPE] = content_type
diff --git a/aiohttp/web_fileresponse.py b/aiohttp/web_fileresponse.py
index 7a29c767ba5..eafc3b051cd 100644
--- a/aiohttp/web_fileresponse.py
+++ b/aiohttp/web_fileresponse.py
@@ -2,6 +2,7 @@
 import io
 import os
 import pathlib
+import sys
 from contextlib import suppress
 from mimetypes import MimeTypes
 from stat import S_ISREG
@@ -360,9 +361,11 @@ async def _prepare_open_file(
         # extension of the request path. The encoding returned by guess_type
         #  can be ignored since the map was cleared above.
         if hdrs.CONTENT_TYPE not in self.headers:
-            self.content_type = (
-                CONTENT_TYPES.guess_type(self._path)[0] or FALLBACK_CONTENT_TYPE
-            )
+            if sys.version_info >= (3, 13):
+                guesser = CONTENT_TYPES.guess_file_type
+            else:
+                guesser = CONTENT_TYPES.guess_type
+            self.content_type = guesser(self._path)[0] or FALLBACK_CONTENT_TYPE
 
         if file_encoding:
             self.headers[hdrs.CONTENT_ENCODING] = file_encoding