Vaa3DTeraFly.wiki

Protocol for Tracing Neurons Using Vaa3D TeraFly

To download Vaa3D, and for the latest information & help visit the Vaa3D website at http://vaa3d.org

This protocol was written to familiarize new users with the Vaa3D TeraFly plug in, which allows for the visualization and analysis of large-scale multidimensional images. In this protocol we describe how to open an image series using TeraFly, offer tips on how to manually trace neurons, and explain how to identify and resolve common tracing errors. The goal of this protocol is to simultaneously accelerate the reconstruction process and reduce the number of errors made during neuron tracing.

Launching TeraFly and Uploading an Image Series:

1. Download Vaa3D from the website mentioned above, then double-click on the vaa3d_msvc.exe file in order to launch the program
2. From the Vaa3D menu bar, select Advanced > Big-Image-Data > TeraFly in order to launch the TeraFly plug-in