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Schedule/Syllabus

Syllabus

Day 1

  • [Course overview and introduction to nanopore sequencing]({{ site.baseurl }}/lectures/overview-intro-nanopore)
  • [Considerations for high molecular weight DNA extraction and library preparation]({{ site.baseurl }}/lectures/DNA-extract-library-prepare)
  • [Principles and practice of modified base detection]({{ site.baseurl }}/lectures/modified-base-detection)
  • [Hands on: Basecalling, sequence alignment, and quality control]({{ site.baseurl }}/materials/sequence-align-pycoqc)
  • [Hands on: Base modification detection]({{ site.baseurl }}/materials/base-mod-detection)
  • [Hands on: Visualisation of base modification data - part I]({{ site.baseurl }}/materials/genome-browser-visualization)

Day 2

  • [Hands on: Manipulation of base modification data]({{ site.baseurl }}/materials/manipulate-modified-base)
  • [Case study: Using nanopore sequencing to study DNA replication dynamics]({{ site.baseurl }}/lectures/case-study)
  • [Hands on: Alternative method for single molecule base modification detection]({{ site.baseurl }}/materials/compare-modification-detection)
  • [Hands on: Visualisation of base modification data - part II]({{ site.baseurl }}/materials/single-molecule-visualization)
  • [Review modification detection pipeline and Q & A]({{ site.baseurl }}/lectures/review-and-qna)