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Add preprocessing steps to the JupyterBook documentation and Readme file #74

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leilaicruz opened this issue Feb 22, 2022 · 0 comments

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leilaicruz commented Feb 22, 2022 •

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Add the necessary preprocessing steps that are needed to perform after getting the raw data from the sequencing company and before input them into the pipeline.
- extracting the forwards reads (if we have paired end sequencing data)
- right trim after the first restriction site sequence for NlaIII and DpnII , and discard the reads that after trimming are less than 100bp

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Include DpnII and NlaIII digestion site trimming #73

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