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commands.conf
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### Example of a run_syny.pl configuration file
### Edit / comment out/ comment in options as desired
################################################################################
### General options
################################################################################
### GenBank GBF/GBFF Annotation files (GZIP files are supported)
# --annot *.gbff.gz
### Output directory [Default = SYNY]
--outdir ./SYNY
### Number of threads to use [Default: 16]
--threads 16
### Number of graphs to plot in parralel; defaults to --threads if unspecified
# --pthreads 8
### DIAMOND BLASTP evalue cutoff [Default = 1e-10]
--evalue 1e-10
### Allowable number of gaps between gene pairs,e.g. --gaps 0, 1, 5 [Default = 0]
--gaps 0
### Minimum contig size (in bp) [Default: 1]; i.e. investigates all contigs
--minsize 1
### Select contigs with names from input text file(s) (one name per line); i.e. exclude everything else
# --include names.txt
### Select contigs with subranges from input text file(s): name start end
# --ranges ranges.txt
### Exclude contigs with names matching the regular expression(s); e.g. --exclude
# --exclude '^AUX'
### Specify genome alignment tool: minimap or mashmap [Default: minimap]
--aligner minimap
### Specify minimap2 max divergence preset (--asm 5, 10 or 20) [Default: off]
# --asm 20
### Specify mashmap percentage identity [Default: 85]
--mpid 85
### Resume minimap2 computations (skip completed alignments)
# --resume
### Skip minimap2 pairwise genome alignments
### --no_map
### Skip minimap VCF file creation (files can be quite large)
### --no_vcf
### Skip gene cluster reconstructions
# --no_clus
################################################################################
### Plotting options
################################################################################
##########################################
# Circos
##########################################
### Circos plot mode: pair (pairwise), cat (concatenated), all (cat + pair) [Default: pair]
--circos pair
### Karyotype orientation: normal, inverted or both [Default: normal]
--orientation normal
### Prefix for concatenated plots [Default: circos]
--circos_prefix circos
### Reference to use for concatenated plots; uses first genome (alphabetically) if ommitted
# --ref reference_name
### Size unit (Kb or Mb) [Default: Mb]
--unit Mb
### Sliding windows size (nucleotide biases) [Default: 10000]
--winsize 10000
### Sliding windows step (nucleotide biases) [Default: 5000]
--stepsize 5000
### Contig label type: mixed (arabic + roman numbers), arabic, roman, or names [Default: mixed]
--labels mixed
### Contig label size [Default: 36]
--label_size 36
### Contig label font [Default: bold]. Possible fonts are: light, normal, default, semibold, bold, italic, bolditalic, italicbold
--label_font bold
### Set max number of ticks [Default: 5000]
--max_ticks 5000
### Set max number of ideograms [Default: 200]
--max_ideograms 200
### Set max number of links [Default: 75000]
--max_links 75000
### Set max number of points per track [Default: 75000]
--max_points_per_track 75000
### Load custom colors from file
# --custom_file custom_color_2.txt
### List available custom color presets
# --list_preset
### Use a custom color preset, e.g.: --custom_preset chloropicon
# --custom_preset chloropicon
### Color by cluster instead of contig/chromosome [Default: off]
# --clusters
### Turn off nucleotide biases subplots
# --no_ntbiases
### Turn off GC / AT skews subplots
# --no_skews
### Turn off ticks in Circos plots
# --no_cticks
### Turn off Circos plots
# --no_circos
##########################################
# Barplots
##########################################
### Barplot figure height in inches [Default: 10.8]
--bheight 10.8
### Barplot figure width in inches [Default: 19.2]
--bwidth 19.2
### Barplot font size [Default: 8]
--bfsize 8
### Barplot color palette [Default: Spectral]
--palette Spectral
### Use a monochrome barplot color instead: e.g. --monobar blue
# --monobar blue
### Color by cluster alternating colors; colors are not related within/between contigs; [Default: off]
# --bclusters
### Barplot mode: pair (pairwise), cat (concatenated), all (cat + pair) [Default: pair]
--bpmode pair
### Turn off barplots
# --no_barplot
##########################################
# Dotplots
##########################################
### Dotplot figure height in inches [Default: 10.8]
--dheight 10.8
### Dotplot figure width in inches [Default: 19.2]
--dwidth 19.2
### Dotplot font size [Default: 8]
--dfsize 8
### Axes units multiplier (for dotplots) [Default: 1e5]
--multi 1e5
### Dotplot color [Default: blue]
--color blue
### Horizontal distance (width) between subplots [Default: 0.05]
--wdis 0.05
### Vertical distance (height) between subplots [Default: 0.1]
--hdis 0.1
### Use a color palette instead: e.g. --dotpalette inferno
# --dotpalette inferno
### Turn off ticks on x and y axes
# --noticks
### Turn off dotplots
# --no_dotplot
##########################################
# Heatmaps
##########################################
### Heatmap figure height in inches [Default: 10]
--hheight 10
### Heatmap figure width in inches [Default: 10]
--hwidth 10
### Heatmap font size [Default: 8]
--hfsize 8
### Heatmap color palette [Default: winter_r]
--hmpalette winter_r
### Set maximum color bar value [Default: 100]
--hmax 100
### Set minimum color bar value [Default: 0]
--hmin 0
### Set color bar values automatically instead
# --hauto
### Turn off heatmaps
# --no_heatmap
##########################################
# Linemaps
##########################################
### Linear map figure height in inches [Default: 5]
--lheight 5
### Heatmap figure width in inches [Default: 20]
--lwidth 20
### Reference genome color palette [Default: Spectral]
--lm_rpalette Spectral
### Target genome color palette [Default: Blues]
--lm_xpalette Blues
### Contig name rotation [Default: 90]
--lmrotation 90
### Font size [Default: 8]
--lfsize 8
### Turn off linemaps
# --no_linemap