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COVID19 Genomics

Data for publications related genomic epidemiology for the Victorian SARS-CoV-2 response from The Peter Doherty Institute for Infection and Immunity and the Victorian Department of Health and Human Services in Australia.

Reads

Raw genome sequence reads have been deposited at NCBI SRA within PRJNA613958. Every isolate was sequenced with Illumina paired-end 150bp reads. Some isolates have additional Nanopore data.

The GISAID ID for each set of reads has been added to the BioSample description.

Consensus genomes

Early versions of consensus genomes were deposited in GISAID, but some were rejected due to "frame shifts". NCBI also rejected them for the same reason. Investigation is ongoing to confirm if these are genuine indels or sequencing artifacts.

Download a multi-FASTA file VIC.ffn of all genomes.

Alignment

Download the aligned-FASTA file VIC.afa

Tree

Download Newick file VIC.nwk

Metadata

Download CSV metadata file VIC.csv

Columns include:

  • VIC_ID - the identifier used in FASTA, AFA and NWK.
  • NCBI_ID - this BioSample ID will link to FASTQ and FASTA
  • GISAID_ID - consensus genome accession
  • Date_coll - date sample was collected
  • Patient_age - patient age in years
  • Patient_sex - patient gender
  • Seq_protocol - ARTIC V1 or V3
  • Sequencing_technology - Instrument sequencing was done on
  • PCR_Ct_value - sample Ct, higher is worse

Citation

If you use any of the data in this repository or PRJNA613958 please cite:

T Seemann, CR Lane, NL Sherry, S Duchene, AG da Silva, L Caly, ML Sait, SA Ballard, K Horan, MB Schultz, T Hoang, M Easton, S Dougall, TP Stinear, J Druce, M Catton, B Sutton, A van Diemen, C Alpren, DA Williamson, BP Howden. Tracking the COVID-19 pandemic in Australia using genomics, medRχiv, 16 May 2020. doi:10.1101/2020.05.12.20099929