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help.nf
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nextflow.enable.dsl=2
def printHeader() {
log.info """\
SINGLE CELL R N A S E Q - N F P I P E L I N E
===============================================
"""
}
def helpMessage() {
yellow = "\033[0;37m"
blue = "\033[0;35m"
white = "\033[0m"
red = "\033[0;31m"
// log.info printHeader()
log.info"""
Usage:
The typical command for running the pipeline is as follows:
${blue} nextflow run main.nf -profile test_local
${yellow}
Mandatory arguments:
--readsfile [csv] Path to the csv file containing list of fastq files
References
--star_index [folder] Specific star index folder that is not among the genomes.
--whitelist [file] File containing a list of valid cell barcodes
OTHER
-- output_dir [folder] Path to the output folder [Default: results]
-- aligner [str] Choose aligner (kallisto,cellranger, etc) [default:star]
-- skip_fastqc [bool] Determine weather to use skipp fastqc or not [Default: null]
Important information:
${red}
""".stripIndent()
}
workflow {
printHeader()
helpMessage()
}