diff --git a/website/docs/user_guide.md b/website/docs/user_guide.md index 71d99b1044..fcd7a7aa09 100644 --- a/website/docs/user_guide.md +++ b/website/docs/user_guide.md @@ -306,10 +306,37 @@ JBrowse uses the same color scheme as IGV for coloring by pair orientation. These pair orientations can be used to reveal complex patterns of structural variation -See https://software.broadinstitute.org/software/igv/interpreting_pair_orientations for a good guide on interpreting these pair orientations +See +https://software.broadinstitute.org/software/igv/interpreting_pair_orientations +for a good guide on interpreting these pair orientations
+### Sashimi-style arcs + +The alignments track will draw sashimi-track style arcs across spliced +alignments (indicated by N in the CIGAR string). If the reads additionally are +tagged with XS tags, it will try to draw the arcs using the strand indicated by +the alignment + +
+ +Note that you can disable these by clicking on the track menu (vertical ... +next to track label, then hovering over SNPCoverage options, and unchecking +"Draw arcs") + +### Insertion and clipping indicators + +The alignments track will also draw a upside-down histogram of insertion and +soft/hard clipped read counts at all positions, and mark significant positions +(covering 30% of the reads) with a purple triangle. + +
+ +Note that you can disable these by clicking on the track menu (vertical ... +next to track label, then hovering over SNPCoverage options, and unchecking +"Draw insertion/clipping indicators" and "Draw insertion/clipping counts") + ## BigWig tracks Visualizing genome signals, whether it is read depth-of-coverage or other @@ -319,24 +346,24 @@ signal, can often be done by using BigWig files ### Viewing whole-genome coverage for profiling CNV -The latest jbrowse also allows refining the resolution of BigWig -tracks, and viewing whole genome coverage. This allows us to get detailed -global views of CNV for example from whole-genome coverage profiling +The latest jbrowse also allows refining the resolution of BigWig tracks, and +viewing whole genome coverage. This allows us to get detailed global views of +CNV for example from whole-genome coverage profiling -Here is a short picture guide to setup a whole-genome view of a BigWig -for CNV coverage visualization +Here is a short picture guide to setup a whole-genome view of a BigWig for CNV +coverage visualization 1. Open your BigWig track 2. Go to the view menu and select "Show all assembly regions" -3. Adjust the "Autoscale type" to your liking, the new options for "Local - +/- 3sd" allows the autoscaling to avoid outliers -4. Go to the track menu and select "Turn off histogram fill", which then - shows only a dot for each point on the graph -5. Go to the track menu and select "Resolution->Finer resolution" a - couple times until resolution looks nice - -Also note: all tracks have a drag handle on the bottom of it which you -can drag down to make the track taller +3. Adjust the "Autoscale type" to your liking, the new options for "Local +/- + 3sd" allows the autoscaling to avoid outliers +4. Go to the track menu and select "Turn off histogram fill", which then shows + only a dot for each point on the graph +5. Go to the track menu and select "Resolution->Finer resolution" a couple + times until resolution looks nice + +Also note: all tracks have a drag handle on the bottom of it which you can drag +down to make the track taller
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