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Releases: BIMSBbioinfo/pigx_rnaseq

Version 0.0.8

04 Jul 08:58
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  • Replaced htseq-count with GenomicAlignments::summarizeOverlaps

    • Now it is faster to count reads
    • The configuration of read counting step is moved to settings.yaml file under
      a new section: "counting"
  • star_map and star_index configuration is now split into two (#55)

  • sorting of BAM files is now done using samtools sort (replaced sorting by STAR) (#51)

Version 0.0.7

20 Jun 14:38
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New Features

  • replaced counts from STAR with ht-seq counts (fixes issue #34 about strandedness)
    • now it is possible to configure counting modes, strandedness, feature types,
      file formats and any other configuration possible using htseq-count.
  • reorganized counts folder
    • redirect all count tables (from htseq-count or salmon or star) to a
      common folder ("feature_counts").
  • creates normalized counts table for all samples using deseq2::estimateSizeFactors
  • Genome coverage (bigwig files) is now normalized using deseq2 size factors.

Fixes issue #34

Version 0.0.6

12 Jun 09:47
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Replaced bedgraph with strand-specific bigwig files for genome coverage.

Fixed bug related to STAR version 2.7.0

Updated instructions for Conda environments.

Fixed issue #52

Version 0.0.5

04 Dec 15:17
v0.0.5
093fe02
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  • Fixed build with Snakemake 5
  • Fixed bug #43
  • Fixed bug #44

Version 0.0.4

02 Jul 05:07
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Version 0.0.3.

20 Mar 11:15
v0.0.3
a366b74
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v0.0.3

Version 0.0.3.

Version 0.0.2

07 Mar 19:07
v0.0.2
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v0.0.2

Release 0.0.2.