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Vardict indicated an ALT depth 2 for an SNV however I confirm multiple ways: pysam, IGV, bcftools mpileup; and all indicate there is only one count for a T in a C > T SNV mutation. I even included ambiguous reads in bcftools mpileup; and same thing. Let me know if I can send a bam file of all the reads for this SNV. It would be 18676 reads for deep sequencing project. Thanks
Brian
The text was updated successfully, but these errors were encountered:
Hi,
Vardict indicated an ALT depth 2 for an SNV however I confirm multiple ways: pysam, IGV, bcftools mpileup; and all indicate there is only one count for a T in a C > T SNV mutation. I even included ambiguous reads in bcftools mpileup; and same thing. Let me know if I can send a bam file of all the reads for this SNV. It would be 18676 reads for deep sequencing project. Thanks
Brian
The text was updated successfully, but these errors were encountered: