diff --git a/module-cheatsheets/scRNA-seq-advanced-cheatsheet.md b/module-cheatsheets/scRNA-seq-advanced-cheatsheet.md index a6ef5af2..3f6c414a 100644 --- a/module-cheatsheets/scRNA-seq-advanced-cheatsheet.md +++ b/module-cheatsheets/scRNA-seq-advanced-cheatsheet.md @@ -1,9 +1,9 @@ # Advanced scRNA-seq Cheatsheet -#### The tables below consist of valuable functions or commands that will help you through this module. -#### Each table represents a different library/tool and its corresponding commands. +### The tables below consist of valuable functions or commands that will help you through this module. +### Each table represents a different library/tool and its corresponding commands. -##### You may also be interested in the following additional cheatsheets: +#### You may also be interested in the following additional cheatsheets: - Download the PDF for the [Introduction to R and Tidyverse cheatsheet](https://github.com/AlexsLemonade/training-modules/raw/master/module-cheatsheets/intro-to-R-tidyverse-cheatsheet.pdf) - Download the PDF for the [Introduction to Single-Cell RNA sequencing cheatsheet](https://github.com/AlexsLemonade/training-modules/raw/master/module-cheatsheets/scRNA-seq-cheatsheet.pdf) @@ -30,12 +30,14 @@ If you have a different version of R or other R packages, the documentation may - [`Seurat` and `SCE` object conversion](#seurat-and-sce-object-conversion) - [Converting from `Seurat` to `SCE`](#converting-from-seurat-to-sce) - [Converting from `SCE` to `Seurat`](#converting-from-sce-to-seurat) + - [Approaches from `ScPCA`](#approaches-from-scpca) +
-### `scater` +## `scater` Read the [`scater` package documentation](https://rdrr.io/bioc/scater/), and a [vignette on its usage](http://www.bioconductor.org/packages/release/bioc/vignettes/scater/inst/doc/overview.html). @@ -48,7 +50,7 @@ Read the [`scater` package documentation](https://rdrr.io/bioc/scater/), and a [ | `scater`| [`plotExpression()`](https://rdrr.io/bioc/scater/man/plotExpression.html)| Plot expression | Plot expression values for all cells in a `SingleCellExperiment` object, using the `logcounts` assay by default| -### `miQC` +## `miQC` Read the [`miQC` package documentation](https://rdrr.io/github/greenelab/miQC/), and a [vignette on its usage](https://www.bioconductor.org/packages/release/bioc/vignettes/miQC/inst/doc/miQC.html). @@ -62,7 +64,7 @@ Read the [`miQC` package documentation](https://rdrr.io/github/greenelab/miQC/), | `miQC`| [`plotFiltering()`](https://rdrr.io/github/greenelab/miQC/man/plotFiltering.html)| Plot filtering | Plot percent of mitochondrial reads against the number of unique genes found, coloring points based on whether they will be filtered out or not | -### `batchelor` and `harmony` +## `batchelor` and `harmony` Read the [`batchelor` package documentation](https://rdrr.io/cran/batchelor/), and a [vignette on its usage](https://rdrr.io/bioc/batchelor/f/vignettes/correction.Rmd). @@ -75,7 +77,7 @@ Read the [`harmony` package documentation](https://rdrr.io/github/immunogenomics | `harmony`| [`HarmonyMatrix()`](https://rdrr.io/github/immunogenomics/harmony/man/HarmonyMatrix.html)| Perform `harmony` integration on a matrix | Perform integration with `harmony` on either a matrix of principle components or gene expression, returning a matrix of batch-corrected principal components | -### `pheatmap` and `EnhancedVolcano` +## `pheatmap` and `EnhancedVolcano` Read the [`pheatmap` package documentation](https://rdrr.io/cran/pheatmap/). Read the [`EnhancedVolcano` package documentation](https://rdrr.io/bioc/EnhancedVolcano/), and [vignette on its usage](https://bioconductor.org/packages/release/bioc/vignettes/EnhancedVolcano/inst/doc/EnhancedVolcano.html). @@ -87,13 +89,13 @@ Read the [`EnhancedVolcano` package documentation](https://rdrr.io/bioc/Enhanced -### `tidyverse` functions +## `tidyverse` functions -#### `purrr` functions +### `purrr` functions -Read the [`purrr` package documentation](https://purrr.tidyverse.org/) and a [vignette on its usage](https://purrr.tidyverse.org/articles/base.html), and download the [`purr` package cheatsheet](https://github.com/rstudio/cheatsheets/blob/main/purrr.pdf). +Read the [`purrr` package documentation](https://purrr.tidyverse.org/) and a [vignette on its usage](https://purrr.tidyverse.org/articles/base.html), and download the [`purr` package cheatsheet](https://github.com/rstudio/cheatsheets/raw/main/purrr.pdf). | Library/Package| Piece of Code| What it's called| What it does | |----------------|--------------|-----------------|--------------| @@ -123,9 +125,9 @@ purrr::map( ``` -#### `ggplot2` functions +### `ggplot2` functions -Read the [`ggplot2` package documentation](https://ggplot2.tidyverse.org/) and an [overall reference for `ggplot2` functions](https://ggplot2.tidyverse.org/reference/index.html), and download the [`ggplot2` package cheatsheet](https://github.com/rstudio/cheatsheets/blob/main/data-visualization.pdf). +Read the [`ggplot2` package documentation](https://ggplot2.tidyverse.org/) and an [overall reference for `ggplot2` functions](https://ggplot2.tidyverse.org/reference/index.html), and download the [`ggplot2` package cheatsheet](https://github.com/rstudio/cheatsheets/raw/main/data-visualization.pdf). | Library/Package| Piece of Code| What it's called| What it does | |----------------|--------------|-----------------|--------------| @@ -140,13 +142,13 @@ Read the [`ggplot2` package documentation](https://ggplot2.tidyverse.org/) and a -#### `dplyr`, `tidyr`,`stringr`, and `tibble` functions +### `dplyr`, `tidyr`,`stringr`, and `tibble` functions Read the full documentation and download cheatsheets (where available) for these `tidyverse` packages at the following links: -- [`dplyr` documentation](https://dplyr.tidyverse.org/) and [`dplyr` cheatsheet](https://github.com/rstudio/cheatsheets/blob/main/data-transformation.pdf) -- [`tidyr` documentation](https://tidyr.tidyverse.org/) and [`tidyr` cheatsheet](https://github.com/rstudio/cheatsheets/blob/main/tidyr.pdf) -- [`stringr` documentation](https://stringr.tidyverse.org/) and [`stringr` cheatsheet](https://github.com/rstudio/cheatsheets/blob/main/strings.pdf) +- [`dplyr` documentation](https://dplyr.tidyverse.org/) and [`dplyr` cheatsheet](https://github.com/rstudio/cheatsheets/raw/main/data-transformation.pdf) +- [`tidyr` documentation](https://tidyr.tidyverse.org/) and [`tidyr` cheatsheet](https://github.com/rstudio/cheatsheets/raw/main/tidyr.pdf) +- [`stringr` documentation](https://stringr.tidyverse.org/) and [`stringr` cheatsheet](https://github.com/rstudio/cheatsheets/raw/main/strings.pdf) - [`tibble` documentation](https://tibble.tidyverse.org/)